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ESIprot enables the charge state determination and molecular weight calculation for low resolution electrospray ionization (ESI) mass spectrometry (MS) data of proteins.

Electrospray ionization (ESI) mass spectrometry (MS) devices with relatively low resolution are widely used for proteomics and metabolomics. Ion trap devices like the Agilent MSD/XCT ultra or the Bruker HCT ultra are typical representatives. However, even if ESI-MS data of most of the naturally ocurring proteins can be measured, the availability of data evaluation software for such ESI protein spectra with low resolution is quite limited.

Main characteristics of ESIprot

Screenshot of ESIprot


Winkler R, ESIprot: A universal tool for charge state determination and molecular weight calculation of proteins from electrospray ionization mass spectrometry data, Rapid Communications in Mass Spectrometry, 2010, 24 (3), 285-294,


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Windows Installer

Python Source Code (zip for Python 2 and 3) (GPLv3).

Git Repository:

You also can use ESIProt Online (without local installation!).